Background Biology
Bacterial Transformation
11 steps of background science. Read them in order, or jump to a step that you need.
Genetic transformation happens when new genetic material is taken up by a cell. The new genetic material often provides the organism with a… In this practical we introduce the pGLO plasmid into a non-pathogenic strain of E. coli As a control we also have bacteria which we do not… We use a technique called heat shock to make the bacteria take up the plasmid. The pGLO plasmid confers two important traits to the bacteria. First of all it allows them to glow green (by the production of Green… The gene for Green Fluorescent Protein is under control of an operon called the arabinose operon. This means that the gene is ONLY switched… The +pGLO bacteria and -pGLO bacteria are grown on plates which contain a jelly-like substance called LB agar. LB agar is a nutrient agar… The bacteria are spread onto the agar. 1) -pGLO bacteria grown on LB agar
These bacteria grow as normal, they do not glow green as they do not have the pGLO plasmid. 2) -pGLO bacteria grown on LB agar containing ampicillin.
These bacteria are all killed by the antibiotic ampicillin. They do not have the… 3) +pGLO bacteria grown on LB agar containing ampicillin.
These bacteria have the pGLO plasmid therefore they can grow on ampicillin.… 4) +pGLO bacteria grown on LB agar containing ampicillin and arabinose.
These bacteria have the pGLO plasmid therefore they can grow on…